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A SummarizedExperiment object containing DIA proteomics data from a UPS1-spiked E. coli proteins, processed using both Spectronaut and ScaffoldDIA separately, then merged.

Format

An instance of the SummarizedExperiment class with the following assays:

norm

This assay includes log2 protein intensities calculated by averaging the peptides derived from the same protein

The object also contains colData and rowData:

colData

A DataFrame with metadata for samples.

rowData

A DataFrame with metadata for proteins.

The colData contains the following columns:

SampleID

Unique identifier for each sample.

Conc

Experimental condition or group for each sample , representing different conc. of UPS1-spiked proteins.

tool

software tool used, Spectronaut or ScaffoldDIA.

fake.batch

A fake digestion batch.

Source

Generated with both spectronaut and ScaffoldDIA separately. using a mixed mode acquisition method and FASTA mode for demonstration purposes.

References

Gotti, C., Roux-Dalvai, F., Joly-Beauparlant, C., Mangnier, L., Leclercq, M., & Droit, A. (2022). DIA proteomics data from a UPS1-spiked E.coli protein mixture processed with six software tools. In Data in Brief (Vol. 41, p. 107829). Elsevier BV. https://doi.org/10.1016/j.dib.2022.107829